Stephen Charles Kowalczykowski Clow, J Krupp, Ra
Published in
Proceedings of the National Academy of Sciences
We have investigated the double-stranded DNA (dsDNA)-dependent ATPase activity of recA protein. This activity is distinguished from the single-stranded DNA (ssDNA)-dependent ATPase activity by the presence of a pronounced lag time before the onset of steady-state ATP hydrolysis. During the lag phase there is little ATP hydrolysis. The duration of t...
Sugiyama, T New, Jh Stephen Charles Kowalczykowski
Published in
Proceedings of the National Academy of Sciences
Homologous recombination in Saccharomyces cerevisiae depends critically on RAD52 function. In vitro, Rad52 protein preferentially binds single-stranded DNA (ssDNA), mediates annealing of complementary ssDNA, and stimulates Rad51 protein-mediated DNA strand exchange. Replication protein A (RPA) is a ssDNA-binding protein that is also crucial to the ...
Cannavo, E Cejka, P Stephen Charles Kowalczykowski
Published in
Proceedings of the National Academy of Sciences
Homologous recombination is a major pathway for repair of DNA double-strand breaks. This repair process is initiated by resection of the 5′-terminated strand at the break site. In yeast, resection is carried out by three nucleolytic complexes: Mre11-Rad50-Xrs2, which functions at the initial step and also stimulates the two processive pathways, Sgs...
Roman, Lj Dixon, Da Stephen Charles Kowalczykowski
Published in
Proceedings of the National Academy of Sciences
We describe the formation of homologously paired joint molecules in an in vitro reaction that is dependent on the concerted actions of purified RecA and RecBCD proteins and is stimulated by single-stranded DNA-binding protein (SSB). RecBCD enzyme initiates the process by unwinding the linear double-stranded DNA to produce single-stranded DNA, which...
Av, Nimonkar Ra, Sica Sc, Kowalczykowski
Published in
Proceedings of the National Academy of Sciences
Saccharomyces cerevisiae Rad52 performs multiple functions during the recombinational repair of double-stranded DNA (dsDNA) breaks (DSBs). It mediates assembly of Rad51 onto single-stranded DNA (ssDNA) that is complexed with replication protein A (RPA); the resulting nucleoprotein filament pairs with homologous dsDNA to form joint molecules. Rad52 ...
Naofumi Handa Yang, L Dillingham, Ms Kobayashi, I Wigley, Db Stephen Charles Kowalczykowski
Published in
Proceedings of the National Academy of Sciences
The RecBCD enzyme is important for both restriction of foreign DNA and recombinational DNA repair. Switching enzyme function from the destructive antiviral state to the productive recombinational state is regulated by the recombination hotspot, χ (5 -GCTGGTGG-3 ). Recognition of χ is unique in that it is recognized as a specific sequence within sin...
Rad, Behzad Forget, Anthony L Baskin, Ronald J Kowalczykowski, Stephen C
Published in
Proceedings of the National Academy of Sciences of the United States of America
DNA helicases are motor proteins that unwind double-stranded DNA (dsDNA) to reveal single-stranded DNA (ssDNA) needed for many biological processes. The RecQ helicase is involved in repairing damage caused by DNA breaks and stalled replication forks via homologous recombination. Here, the helicase activity of RecQ was visualized on single molecules...
Chen, H Donnianni, Ra Naofumi Handa Deng, Sk Oh, J Timashev, La Stephen Charles Kowalczykowski Symington, Ls
Published in
Proceedings of the National Academy of Sciences
The Mre11-Rad50-Xrs2/NBS1 (MRX/N) nuclease/ATPase complex plays structural and catalytic roles in the repair of DNA double-strand breaks (DSBs) and is the DNA damage sensor for Tel1/ATM kinase activation. Saccharomyces cerevisiae Sae2 can function with MRX to initiate 5 -3 end resection and also plays an important role in attenuation of DNA damage...
Katsumi Morimatsu Stephen Charles Kowalczykowski
Published in
Proceedings of the National Academy of Sciences
Recombinational DNA repair by the RecF pathway of Escherichia coli requires the coordinated activities of RecA, RecFOR, RecQ, RecJ, and single-strand DNA binding (SSB) proteins. These proteins facilitate formation of homologously paired joint molecules between linear double-stranded (dsDNA) and supercoiled DNA. Repair starts with resection of the b...
Bianco, Pr Stephen Charles Kowalczykowski
Published in
Proceedings of the National Academy of Sciences
The RecBCD enzyme of Escherichia coli functions in the seemingly disparate roles of homologous recombination and the degradation of DNA. Which of these two roles it assumes is regulated by the 8-base recombination hotspot, Chi. Using double-stranded DNA substrates that are heteroduplex at the Chi locus we have established the determinants for Chi r...
