Hilario, Jovencio Amitani, Ichiro Baskin, Ronald J Kowalczykowski, Stephen C
Published in
Proceedings of the National Academy of Sciences of the United States of America
Rad51 protein (Rad51) is central to recombinational repair of double-strand DNA breaks. It polymerizes onto DNA and promotes strand exchange between homologous chromosomes. We visualized the real-time assembly and disassembly of human Rad51 nucleoprotein filaments on double-stranded DNA by single-molecule fluorescence microscopy. Rad51 assembly ext...
Av, Nimonkar Az, Ozsoy J, Genschel P, Modrich Sc, Kowalczykowski
Published in
Proceedings of the National Academy of Sciences
The error-free repair of double-stranded DNA breaks by homologous recombination requires processing of broken ends. These processed ends are substrates for assembly of DNA strand exchange proteins that mediate DNA strand invasion. Here, we establish that human BLM helicase, a member of the RecQ family, stimulates the nucleolytic activity of human e...
Av, Nimonkar Ra, Sica Sc, Kowalczykowski
Published in
Proceedings of the National Academy of Sciences
Saccharomyces cerevisiae Rad52 performs multiple functions during the recombinational repair of double-stranded DNA (dsDNA) breaks (DSBs). It mediates assembly of Rad51 onto single-stranded DNA (ssDNA) that is complexed with replication protein A (RPA); the resulting nucleoprotein filament pairs with homologous dsDNA to form joint molecules. Rad52 ...
Rad, Behzad Forget, Anthony L Baskin, Ronald J Kowalczykowski, Stephen C
Published in
Proceedings of the National Academy of Sciences of the United States of America
DNA helicases are motor proteins that unwind double-stranded DNA (dsDNA) to reveal single-stranded DNA (ssDNA) needed for many biological processes. The RecQ helicase is involved in repairing damage caused by DNA breaks and stalled replication forks via homologous recombination. Here, the helicase activity of RecQ was visualized on single molecules...
