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RecBCD enzyme is a bipolar DNA helicase.

Dillingham, Ms Spies, M Stephen Charles Kowalczykowski

Published in Nature

Escherichia coli RecBCD is a heterotrimeric helicase/nuclease that catalyses a complex reaction in which double-strand breaks in DNA are processed for repair by homologous recombination. For some time it has been clear that the RecB subunit possesses a 3 —> 5 DNA helicase activity, which was thought to drive DNA translocation and unwinding in the...

Rad52 protein stimulates DNA strand exchange by Rad51 and replication protein A.

New, Jh Sugiyama, T Zaitseva, E Stephen Charles Kowalczykowski

Published in Nature

The generation of a double-strand break in the Saccharomyces cerevisiae genome is a potentially catastrophic event that can induce cell-cycle arrest or ultimately result in loss of cell viability. The repair of such lesions is strongly dependent on proteins encoded by the RAD52 epistasis group of genes (RAD50-55, RAD57, MRE11, XRS2), as well as the...

Processive translocation and DNA unwinding by individual RecBCD enzyme molecules.

Bianco, Pr Brewer, Lr Corzett, M Balhorn, R Yeh, Y Stephen Charles Kowalczykowski Baskin, Rj

Published in Nature

RecBCD enzyme is a processive DNA helicase and nuclease that participates in the repair of chromosomal DNA through homologous recombination. We have visualized directly the movement of individual RecBCD enzymes on single molecules of double-stranded DNA (dsDNA). Detection involves the optical trapping of solitary, fluorescently tagged dsDNA molecul...

DNA end resection by Dna2-Sgs1-RPA and its stimulation by Top3-Rmi1 and Mre11-Rad50-Xrs2.

Cejka, P Cannavo, E Polaczek, P Masuda-Sasa, T Pokharel, S Campbell, Jl Stephen Charles Kowalczykowski

Published in Nature

The repair of DNA double-strand breaks (DSBs) by homologous recombination requires processing of broken ends. For repair to start, the DSB must first be resected to generate a 3 -single-stranded DNA (ssDNA) overhang, which becomes a substrate for the DNA strand exchange protein, Rad51 (ref. 1). Genetic studies have implicated a multitude of protein...

Translocation step size and mechanism of the RecBC DNA helicase.

Bianco, Pr Stephen Charles Kowalczykowski

Published in Nature

DNA helicases are ubiquitous enzymes that unwind double-stranded DNA. They are a diverse group of proteins that move in a linear fashion along a one-dimensional polymer lattice—DNA—by using a mechanism that couples nucleoside triphosphate hydrolysis to both translocation and double-stranded DNA unwinding to produce separate strands of DNA. The RecB...

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