Kowalczykowski Lab - UC Davis
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Watching Individual Proteins Acting on Single Molecules of DNA

Amitani, Ichiro Liu, Bian Dombrowski, Christopher C. Baskin, Ronald J. Kowalczykowski, Stephen C.

Published in Methods in Enzymology

In traditional biochemical experiments, the behavior of individual proteins is obscured by ensemble averaging. To better understand the behavior of proteins that bind to and/or translocate on DNA, we have developed instrumentation that uses optical trapping, microfluidic solution delivery, and fluorescent microscopy to visualize either individual p...

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