Autoregulation of gene expression. Quantitative evaluation of the expression and function of the bacteriophage T4 gene 3...
Published in Journal of Molecular Biology
Archaeal RadA protein binds DNA as both helical filaments and octameric rings.
Published in Journal of Molecular Biology
The Escherichia coli RecA protein has been a model for understanding homologous eukaryotic recombination proteins such as Rad51. The active form of both RecA and Rad51 appear to be helical filaments polymerized on DNA, in which an unusual helical structure is induced in the DNA. Surprisingly, the human meiosis-specific homolog of RecA, Dmc1, has th...
Negative co-dominant inhibition of recA protein function. Biochemical properties of the recA1, recA13 and recA56 protein...
Published in Journal of Molecular Biology
We have investigated the biochemical properties of several Escherichia coli mutant recA proteins that display a null phenotype. These are the recA1, recA13 and recA56 proteins, each of which carries a single missense mutation. These proteins all share a common defect which is the inability to adopt the high affinity DNA binding state normally elici...
Interaction of recA protein with single-stranded DNA. Quantitative aspects of binding affinity modulation by nucleotide ...
Published in Journal of Molecular Biology
We have investigated quantitative molecular aspects of the interaction of recA protein with single-stranded DNA, by using a fluorescent modified-DNA referred to as etheno-M13 DNA. In addition, the effects of the nucleotide cofactors ATP and ADP, and the analogues ATP-gamma-S, AMP-P-C-P, and AMP-P-N-P on this interaction have been studied. It is sho...
A novel, 11 nucleotide variant of chi, chi*: one of a class of sequences defining the Escherichia coli recombination hot...
Published in Journal of Molecular Biology
In wild-type Escherichia coli, recognition of the recombination hotspot, chi (5 -GCTGGTGG-3 ), by the RecBCD enzyme is central to homologous recombination. However, in the recC* class of RecBCD mutants, stimulation of recombination by the canonical chi sequence is not detectable, but the levels of homologous recombination are nearly wild-type. In v...
Kinetics and mechanism of the association of the bacteriophage T4 gene 32 (helix destabilizing) protein with single-stra...
Published in Journal of Molecular Biology
Biochemical properties of the Escherichia coli recA430 protein. Analysis of a mutation that affects the interaction of t...
Published in Journal of Molecular Biology
The biochemical properties of the recA430 protein have been examined and compared to those of wild-type recA protein. We find that, while the recA430 protein possesses ssDNA-dependent rATP activity, this activity is inhibited by the Escherichia coli single-stranded DNA binding protein (SSB protein) under many conditions that enhance wild-type recA ...
The mutant recBCD enzyme, recB2109CD enzyme, has helicase activity but does not promote efficient joint molecule formati...
Published in Journal of Molecular Biology
The Escherichia coli recB2109CD enzyme displays a defect in homologous recombination. In vitro, it possesses significant levels of non-specific nuclease activity but is deficient in chi-dependent nicking activity. To determine whether an alteration in helicase activity contributes further to its in vivo defect, the ability of recB2109CD enzyme to u...
Interactions of bacteriophage T4-coded gene 32 protein with nucleic acids. I. Characterization of the binding interactio...
Published in Journal of Molecular Biology
A RecA mutant, RecA(730), suppresses the recombination deficiency of the RecBC(1004)D-chi* interaction in vitro and in v...
Published in Journal of Molecular Biology
In Escherichia coli, homologous recombination initiated at double-stranded DNA breaks requires the RecBCD enzyme, a multifunctional heterotrimeric complex that possesses processive helicase and exonuclease activities. Upon encountering the DNA regulatory sequence, chi, the enzymatic properties of RecBCD enzyme are altered. Its helicase activity is ...
