Fluorescent single-stranded DNA binding protein as a probe for sensitive, real-time assays of helicase activity.
Published in Biophysical journal
The formation and maintenance of single-stranded DNA (ssDNA) are essential parts of many processes involving DNA. For example, strand separation of double-stranded DNA (dsDNA) is catalyzed by helicases, and this exposure of the bases on the DNA allows further processing, such as replication, recombination, or repair. Assays of helicase activity and...
