Kowalczykowski Lab - UC Davis
  • Upload

  • Log in

  • Sign up

Search on MyScienceWork
1 News 192 Publications 19 Members

Cooperative and noncooperative binding of protein ligands to nucleic acid lattices: experimental approaches to the deter...

Stephen Charles Kowalczykowski Paul, Ls Lonberg, N Newport, Jw Mcswiggen, Ja Von Hippel, Ph

Published in Biochemistry

Many biologically important proteins bind nonspecifically, and often cooperatively, to single-or double-stranded nucleic acid lattices in discharging their physiological functions. This binding can generally be described in thermodynamic terms by three parameters: n, the binding site size; K, the intrinsic binding constant; omega, the binding coope...

Some assembly required.

Stephen Charles Kowalczykowski

Published in Nautre Structural Biology

The DNA binding properties of Saccharomyces cerevisiae Rad51 protein.

Zaitseva, Em Zaitsev, En Stephen Charles Kowalczykowski

Published in Journal of Biological Chemistry

Saccharomyces cerevisiae Rad51 protein is the paradigm for eukaryotic ATP-dependent DNA strand exchange proteins. To explain some of the unique characteristics of DNA strand exchange promoted by Rad51 protein, when compared with its prokaryotic homologue the Escherichia coli RecA protein, we analyzed the DNA binding properties of the Rad51 protein....

Coupling of DNA helicase function to DNA strand exchange activity.

Harmon, Fg Stephen Charles Kowalczykowski

Published in Methods in Molecular Biology

Purification and characterization of the coliphage N4-coded single-stranded DNA binding protein.

Lindberg, G Stephen Charles Kowalczykowski Rist, Jk Sugino, A Rothman-Denes, Lb

Published in Journal of Biological Chemistry

We have purified and characterized a single-stranded DNA binding protein (N4 SSB) induced after coliphage N4 infection. It has a monomeric molecular weight of 31,000 and contains 10 tyrosine and 1-2 tryptophan amino acid residues. Its fluorescence spectrum is dominated by the tyrosine residues, and their fluorescence is quenched when the protein bi...

Essential monomer-monomer contacts define the minimal length for the N-terminus of RecA protein.

Zaitsev, En Stephen Charles Kowalczykowski

Published in Molecular Microbiology

The DNA binding site(s) of the Escherichia coli RecA protein.

Rehrauer, Wm Stephen Charles Kowalczykowski

Published in Journal of Biological Chemistry

Photochemical cross-linking has been used to identify residues in the Escherichia coli RecA protein that are proximal to and may directly mediate binding of DNA. Ultraviolet irradiation promotes specific and efficient cross-linking of the RecA protein to poly(deoxythymidylic) acid. Cross-linked peptides remaining covalently attached to the polynucl...

The recombination hot spot chi is embedded within islands of preferred DNA pairing sequences in the E. coli genome.

Tracy, Rb Chédin, F Stephen Charles Kowalczykowski

Published in Cell

Autoregulation of gene expression. Quantitative evaluation of the expression and function of the bacteriophage T4 gene 3...

Von Hippel, Ph Stephen Charles Kowalczykowski Lonberg, N Newport, Jw Paul, Ls Stormo, Gd Gold, L

Published in Journal of Molecular Biology

The recombination-deficient mutant RPA (rfa1-t11) is displaced slowly from single-stranded DNA by Rad51 protein.

Kantake, N Sugiyama, T Kolodner, Rd Stephen Charles Kowalczykowski

Published in Journal of Biological Chemistry

Replication protein-A (RPA) is involved in many processes of DNA metabolism, including DNA replication, repair, and recombination. Cells carrying a mutation in the largest subunit of RPA (rfa1-t11: K45E) have defects in meiotic recombination, mating-type switching, and survival after DNA damage caused by UV and methyl methanesulfonate, as well as i...

  • ← Previous
  • 1
  • 2
  • 3
  • 4 (current)
  • 5
  • 6
  • …
  • 19
  • Next →
Powered by Polaris
  • About the Lab
  • Our Research
  • FAQ