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Published in Nautre Structural Biology
The DNA binding properties of Saccharomyces cerevisiae Rad51 protein.
Published in Journal of Biological Chemistry
Saccharomyces cerevisiae Rad51 protein is the paradigm for eukaryotic ATP-dependent DNA strand exchange proteins. To explain some of the unique characteristics of DNA strand exchange promoted by Rad51 protein, when compared with its prokaryotic homologue the Escherichia coli RecA protein, we analyzed the DNA binding properties of the Rad51 protein....
Coupling of DNA helicase function to DNA strand exchange activity.
Published in Methods in Molecular Biology
Purification and characterization of the coliphage N4-coded single-stranded DNA binding protein.
Published in Journal of Biological Chemistry
We have purified and characterized a single-stranded DNA binding protein (N4 SSB) induced after coliphage N4 infection. It has a monomeric molecular weight of 31,000 and contains 10 tyrosine and 1-2 tryptophan amino acid residues. Its fluorescence spectrum is dominated by the tyrosine residues, and their fluorescence is quenched when the protein bi...
Essential monomer-monomer contacts define the minimal length for the N-terminus of RecA protein.
Published in Molecular Microbiology
The DNA binding site(s) of the Escherichia coli RecA protein.
Published in Journal of Biological Chemistry
Photochemical cross-linking has been used to identify residues in the Escherichia coli RecA protein that are proximal to and may directly mediate binding of DNA. Ultraviolet irradiation promotes specific and efficient cross-linking of the RecA protein to poly(deoxythymidylic) acid. Cross-linked peptides remaining covalently attached to the polynucl...
Single-molecule imaging brings Rad51 nucleoprotein filaments into focus.
Published in Trends in Cell Biology
The Rad51 protein is essential for DNA repair by homologous recombination. After DNA damage, Rad51 localizes to nuclear foci that represent sites of DNA repair in vivo. In vitro, Rad51 self-assembles on single- or double-stranded DNA to form a nucleoprotein filament. Recently, the merging of innovative single-molecule techniques with ensemble metho...
The recombination hot spot chi is embedded within islands of preferred DNA pairing sequences in the E. coli genome.
Published in Cell
Autoregulation of gene expression. Quantitative evaluation of the expression and function of the bacteriophage T4 gene 3...
Published in Journal of Molecular Biology
The recombination-deficient mutant RPA (rfa1-t11) is displaced slowly from single-stranded DNA by Rad51 protein.
Published in Journal of Biological Chemistry
Replication protein-A (RPA) is involved in many processes of DNA metabolism, including DNA replication, repair, and recombination. Cells carrying a mutation in the largest subunit of RPA (rfa1-t11: K45E) have defects in meiotic recombination, mating-type switching, and survival after DNA damage caused by UV and methyl methanesulfonate, as well as i...
