Kowalczykowski Lab - UC Davis
  • Upload

  • Log in

  • Sign up

Search on MyScienceWork
1 News 192 Publications 19 Members

Translocation step size and mechanism of the RecBC DNA helicase.

Bianco, Pr Stephen Charles Kowalczykowski

Published in Nature

DNA helicases are ubiquitous enzymes that unwind double-stranded DNA. They are a diverse group of proteins that move in a linear fashion along a one-dimensional polymer lattice—DNA—by using a mechanism that couples nucleoside triphosphate hydrolysis to both translocation and double-stranded DNA unwinding to produce separate strands of DNA. The RecB...

Biochemical basis of the constitutive repressor cleavage activity of recA730 protein. A comparison to recA441 and recA80...

Lavery, Pe Stephen Charles Kowalczykowski

Published in Journal of Biological Chemistry

The recA730 mutation results in constitutive SOS and prophage induction. We examined biochemical properties of recA730 protein in an effort to explain the constitutive activity observed in recA730 strains. We find that recA730 protein is more proficient than the wild-type recA protein in the competition with single-stranded DNA binding protein (SSB...

An Overview of the Molecular Mechanisms of Recombinational DNA Repair.

Stephen Charles Kowalczykowski

Published in Cold Spring Harbor Perspectives in Biology

Recombinational DNA repair is a universal aspect of DNA metabolism and is essential for genomic integrity. It is a template-directed process that uses a second chromosomal copy (sister, daughter, or homolog) to ensure proper repair of broken chromosomes. The key steps of recombination are conserved from phage through human, and an overview of those...

A step backward in advancing DNA replication: rescue of stalled replication forks by RecG.

Dillingham, Ms Stephen Charles Kowalczykowski

Published in Molecular Cell

In the October 5 issue of Cell, Singleton et al. report the crystal structure of RecG protein bound to an analog of a stalled DNA replication fork. This structure shows how RecG can recognize branched DNA structures and suggests a mechanism for fork reversal, an early event in recombination-dependent reinitiation of DNA replication.

The Bacillus subtilis AddAB helicase/nuclease is regulated by its cognate Chi sequence in vitro.

Chédin, F Ehrlich, Sd Stephen Charles Kowalczykowski

Published in Journal of Molecular Biology

The AddAB enzyme is important to homologous DNA recombination in Bacillus subtilis, where it is thought to be the functional counterpart of the RecBCD enzyme of Escherichia coli. In vivo, AddAB responds to a specific five-nucleotide sequence (5 -AGCGG-3 or its complement) in a manner analogous to the response of the RecBCD enzyme to interaction wi...

Biochemical events essential to the recombination activity of Escherichia coli RecA protein. II. Co-dominant effects of ...

Stephen Charles Kowalczykowski Krupp, Ra

Published in Journal of Molecular Biology

In the accompanying paper, RecA142 protein was found to be completely defective in DNA heteroduplex formation. Here, we show that RecA142 protein not only is defective in this activity but also is inhibitory for certain activities of wild-type RecA protein. Under appropriate conditions, RecA142 protein substantially inhibits the DNA strand exchange...

Biochemical events essential to the recombination activity of Escherichia coli RecA protein. I. Properties of the mutant...

Stephen Charles Kowalczykowski Burk, Dl Krupp, Ra

Published in Journal of Molecular Biology

We have characterized the biochemical properties of Escherichia coli RecA142 protein, the product of a recA allele that is phenotypically defective in genetic recombination. In vitro, this mutant RecA protein is totally defective in DNA heteroduplex formation. Despite this defect, RecA142 protein is not deficient in all other biochemical activities...

Saccharomyces cerevisiae Dmc1 and Rad51 proteins preferentially function with Tid1 and Rad54 proteins, respectively, to ... PDF available through Get Fulltext Research

Nimonkar, Av Dombrowski, Cc Siino, Js Stasiak, Az Stasiak, A Stephen Charles Kowalczykowski

Published in Journal of Biological Chemistry

The Saccharomyces cerevisiae Dmc1 and Tid1 proteins are required for the pairing of homologous chromosomes during meiotic recombination. This pairing is the precursor to the formation of crossovers between homologs, an event that is necessary for the accurate segregation of chromosomes. Failure to form crossovers can have serious consequences and m...

The DNA binding preference of RAD52 and RAD59 proteins: implications for RAD52 and RAD59 protein function in homologous ...

Sugiyama, T Stephen Charles Kowalczykowski Wu, Y Siino, Js

Published in Journal of Biological Chemistry

We examined the double-stranded DNA (dsDNA) binding preference of the Saccharomyces cerevisiae Rad52 protein and its homologue, the Rad59 protein. In nuclease protection assays both proteins protected an internal sequence and the dsDNA ends equally well. Similarly, using electrophoretic mobility shift assays, we found the affinity of both Rad52 and...

Molecular determinants responsible for recognition of the single-stranded DNA regulatory sequence, χ, by RecBCD enzyme.

Naofumi Handa Yang, L Dillingham, Ms Kobayashi, I Wigley, Db Stephen Charles Kowalczykowski

Published in Proceedings of the National Academy of Sciences

The RecBCD enzyme is important for both restriction of foreign DNA and recombinational DNA repair. Switching enzyme function from the destructive antiviral state to the productive recombinational state is regulated by the recombination hotspot, χ (5 -GCTGGTGG-3 ). Recognition of χ is unique in that it is recognized as a specific sequence within sin...

  • ← Previous
  • 1
  • …
  • 13
  • 14
  • 15 (current)
  • 16
  • 17
  • 18
  • 19
  • Next →
Powered by Polaris
  • About the Lab
  • Our Research
  • FAQ